Potensi Genistein pada Sistem Reproduksi Mencit sebagai Penyusunan Bahan Ajar Fisiologi Reproduksi

Cicilia Primiani

Abstract


Penelitian ini bertujuan untuk menguji pengaruh genistein terhadap sistem reproduksi mencit jantan (Mus musculus). Memanfaatkan hasil penelitian pengaruh genistein terhadap sistem reproduksi mencit jantan (Mus musculus) sebagai bahan untuk menyusun bahan ajar mata kuliah fisiologi. Penelitian ini menggunakan pendekatan kuantitatif eksperimental, yang rancangannya mengikuti Rancangan Acak Kelompok. Variabel bebas adalah dosis genistein 0 mg/g, 0,0035 mg/g, 0,0042 mg/g, dan 0,0049 mg/g. Hasil penelitian menunjukkan bahwa ada pengaruh genistein pada sistem reproduksi mencit (Mus musculus). Genistein yang diberikan dengan dosis 0,0035 mg/g; 0,0042 mg/g; dan 0,0049 mg/g berpengaruh terhadap jumlah sel germinal dalam tubulus seminiferus testis, morfologi dan viabilitas spermatozoa.

Keywords


Genisetin, Sistem reproduksi, Kuantitatif eksperimental, Rancangan acak kelompok

Full Text:

PDF

References


Abney, T. O., & Myers, R. B. (1991). 17β‐Estradiol Inhibition of Leydig Cell Regeneration in the Ethane Dimethylsulfonate‐Treated Mature Rat. Journal of Andrology, 12(5), 295–304.

Akiyama, T., Ishida, J., Nakagawa, S., Ogawara, H., Watanabe, S. I., Itoh, N., & Fukami, Y. (1987). Genistein, a specific inhibitor of tyrosine-specific protein kinases. Journal of Biological Chemistry, 262(12), 5592–5595.

Alexandrakis, M. G., Kyriakou, D. S., Kempuraj, D., Huang, M., Boucher, W., Seretakis, D., & Theoharides, T. C. (2003). The isoflavone genistein inhibits proliferation and increases histamine content in human leukemic mast cells. Allergy and Asthma Proceedings, 24(5), 373. OceanSide Publications.

Bajpai, M., Asin, S., & Doncel, G. F. (2003). Effect of tyrosine kinase inhibitors on tyrosine phosphorylation and motility parameters in human sperm. Archives of Andrology, 49(3), 229–246.

Barlow, J., Johnson, J. A., & Scofield, L. (2007). Fact sheet on the phytoestrogen genistein. In NIEHS/NCI Environment Research Centers,(Online), http://cerhr. niehs. nih. gov/chemicals/genistein (Vol. 8).

Cardoso, J. R., Mondadori, R. G., Bianchini, E., & Báo, S. N. (2007). Effects of chronic treatment with soy derived isoflavones on reproductive health of male rabbits.

Chavarro, J. E., Toth, T. L., Sadio, S. M., & Hauser, R. (2008). Soy food and isoflavone intake in relation to semen quality parameters among men from an infertility clinic. Human Reproduction, 23(11), 2584–2590.

Delmonte, P., & Rader, J. I. (2006). Analysis of isoflavones in foods and dietary supplements. Journal of AOAC International, 89(4), 1138–1146.

Gruber, C. J., Tschugguel, W., Schneeberger, C., & Huber, J. C. (2002). Production and actions of estrogens. New England Journal of Medicine, 346(5), 340–352.

Handelsman, D. J., Wishart, S., & Conway, A. J. (2000). Oestradiol enhances testosterone-induced suppression of human spermatogenesis. Human Reproduction, 15(3), 672–679.

Harmita, R. M. (2005). Analisis hayati. Jakarta: Percetakan Ari Cipta, 51, 55.

Kuiper, G. G., Lemmen, J. G., Carlsson, B. O., Corton, J. C., Safe, S. H., Van Der Saag, P. T., & Gustafsson, J. A. (1998). Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor β. Endocrinology, 139(10), 4252–4263.

Kuntana, Y. P. (2009). Pengaruh Pemberian Phytoestrogen Terhadap Kualitas Spermatozoa, Spermatogenesis dan Luas Jaringan Interstitial pada Kelinci (Oryctolagus cuniculus). Jurnal Bionatura, 11(1), 47–58.

Lee, B. J., Kang, J. K., Jung, E. Y., Yun, Y. W., Baek, I. J., Yon, J. M., & Nam, S. Y. (2004a). Effects of exposure to genistein during pubertal development on the reproductive system of male mice. Journal of Reproduction and Development, 50(4), 399–409.

Lee, B. J., Kang, J. K., Jung, E. Y., Yun, Y. W., Baek, I. J., Yon, J. M., & Nam, S. Y. (2004b). Exposure to genistein does not adversely affect the reproductive system in adult male mice adapted to a soy-based commercial diet. Journal of Veterinary Science, 5(3), 227–234.

Matsukawa, Y., Marui, N., Sakai, T., Satomi, Y., Yoshida, M., Matsumoto, K., & Aoike, A. (1993). Genistein arrests cell cycle progression at G2-M. Cancer Research, 53(6), 1328–1331.

Opalka, M., Kaminska, B., Ciereszko, R., & Dusza, L. (2004). Genistein affects testosterone secretion by Leydig cells in roosters (Gallus gallus domesticus). Reproductive Biology, 4(2), 185–193.

Primiani, C. N., & Susianingsih, M. D. (2010). Meningkatkan aktivitas dan prestasi belajar biologi melalui pendekatan kontekstual dengan media herbarium dan insektarium. Paedagogia, 13(1).

Setchell, K. D., Zimmer-Nechemias, L., Cai, J., & Heubi, J. E. (1998). Isoflavone content of infant formulas and the metabolic fate of these phytoestrogens in early life. The American Journal of Clinical Nutrition, 68(6), 1453S-1461S.

Shibayama, T., Fukata, H., Sakurai, K., Adachi, T., Komiyama, M., Iguchi, T., & Mori, C. (2001). Neonatal exposure to genistein reduces expression of estrogen receptor alpha and androgen receptor in testes of adult mice. Endocrine Journal, 48(6), 655–663.

Sirivaidyapong, S., & Uthai, S. (2003). Effect of Collection Time and Collection Temperature on Motility and Viability of Canine Epididymal Sperm. International Symposium of The World Association of Veterinary Laboratory Diagnosticians and OIE Seminar on Biotechnology, 9–13.

Visconti, P. E., & Kopf, G. S. (1998). Regulation of protein phosphorylation during sperm capacitation. Biology of Reproduction, 59(1), 1–6.

Widiyanto, J. (2010). SPSS For Windows untuk analisis data statistik dan penelitian. Surakarta: BP-FKIP UMS.




DOI: https://doi.org/10.47213/bp.v1i1.20

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Cicilia Primiani

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Statistik BP


Belantika Pendidikan

Jendela Pendidikan Indonesia

Kayon Media

Lisensi Creative Commons

Belantika Pendidikan is licensed under Creative Commons Attribution-ShareAlike 4.0 International License